Virus:Article Title: Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep anti-SULT1B1 R&D Systems Cat# AF5959; RRID: AB_10571701; Lot:CDWJD12104A Rabbit anti-SULT1B1 Thermo Fisher Scientific Cat# PA5-50796; RRID: AB_2636248; Lot: 30FB4A02 Rabbit anti-H3Y99 Sulfation PTM Biolabs Cat# CTM-211; Lot: ZC199K415P2 Mouse anti-α-Tubulin Abclonal Cat# AC012; RRID: AB_2768341; Lot: 9100012001 Rabbit anti-Histone H3 Abclonal Cat# A2348; RRID: AB_2631273; Lot: 35075 Rabbit anti-PAPSS2 Proteintech Cat# 15978-1-AP; RRID: AB_2159498; Lot: 00041398 Rabbit anti-PAPSS1 Proteintech Cat# 14708-1-AP; RRID: AB_2159487; Lot: 00046631 Rabbit anti-SLC26A1 HUABIO Cat# TP50897; Lot: RB8023 Guinea pig anti-NeuN Oasis biofarm Cat# OB-PGP006-01; RRID: AB_2934234 Guinea pig anti-iba1 Oasis biofarm Cat# OB-PGP049-01; RRID: AB_2934253 Guinea pig anti-GFAP Oasis biofarm Cat# PGP055; RRID: AB_2938933 Guinea pig anti-Olig2 Oasis biofarm Cat# OB-PGP040-02; RRID: AB_2934243 Goat anti-CD31 R&D Systems Cat# AF3628; RRID: AB_2161028 Rabbit anti-ARG1 CST Cat# 93668; RRID: AB_2800207 Rabbit anti-CD86 CST Cat# 19589; RRID: AB_2892094 Goat anti-Guinea pig IgG, 488nm Oasis biofarm Cat# OB-GP488-50; RRID: AB_3717497 Donkey anti-Guinea pig IgG, 594nm Oasis biofarm Cat# D-GP594-50; RRID: AB_3694202 Alexa Fluor®594 donkey anti-rabbit IgG(H + L) Thermo Fisher Cat# A21207; RRID: AB_141637 Alexa Fluor®488 donkey anti-goat IgG(H + L) Thermo Fisher Cat# A11055; RRID: AB_2534102 Rabbit anti-PDK1 Abclonal Cat# A8930; RRID: AB_2863629, Lot: 4000001355 Mouse anti-PFKP Proteintech Cat# 68129-1-Ig; RRID: AB_2923656, Lot: 10029401 Mouse anti-PKM2 Proteintech Cat# 60268-1-Ig; RRID: AB_2881388, Lot: 10024075 Mouse anti-HK2 ProMab Cat# 30088 Bacterial and virus strains Trans1-T1 Phage Resistant Chemically Competent Cell TransGen Biotech Cat# CD501-02 pAAV[shRNA]-CAG>EGFP:T2A:Puro-U6>mPapss1 VectorBuilder Inc. N/A pAAV[shRNA]-EGFP-U6>Scramble_shRNA VectorBuilder Inc. N/A pAAV[Exp]-CAG>mPapss1[NM_011863.3](ns): P2A:EGFP:WPRE VectorBuilder Inc. N/A pAAV[Exp]-CAG>EGFP:WPRE VectorBuilder Inc. N/A Biological samples Human serum samples from patients with ischemic stroke The Affiliated Nanhua Hospital, University of South China N/A Human serum samples from healthy control subjects The Affiliated Nanhua Hospital, University of South China N/A (Continued on next page) e1 Cell Reports Medicine 7, 102684, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMSO VWR Chemicals Cat# 0231-500ML; CAS: 67-68-5, Lot: 22D2056114 Tween 20 BioFroxx Cat# 1247ML500 Fetal bovine serum Pricella Cat# 086–150; Lot: SA240119 DMEM basic (1X) Gibco Cat# SH30285.01; Lot: 6124583 Penicillin-Streptomycin Solution (100X) biosharp Cat# BL505A; Lot: 24208424 0.25% Trypsin-EDTA (1X) Gibco Cat# 25200-072, Lot: 2919428 Phosphatase inhibitor cocktail I MCE Chemexpress Cat# HY-K0021 Phosphatase inhibitor cocktail II MCE Chemexpress Cat# HY-K0022 Protease inhibitor cocktail MCE Chemexpress Cat# HY-K0010 Deacetylase inhibitor cocktail MCE Chemexpress Cat# HY-K0030 Coomassie brilliant blue R-250 BioFroxx Cat# 1912 Trypan Blue Staining Solution (0.4%) Biosharp Cat# BL627A, Lot: 23338403 Methylsulfonylmethane Aladdin CAS: 67-71-0 AgeI-HF BioLabs Cat# R3552L EcoRI-HF BioLabs Cat# R3101S Polyethylenimine Linear (PEI) Yeasen Biotechnology Cat# 40815ES03 2x Phanta Max Master Mix Vazyme Cat# P515-02, Lot: 7E2352K4 Critical commercial assays E.Z.N.A Plasmid Mini Kit I Omega Bio-tek Cat# D6943-02 RNAsimple Total RNA Kit TIANGEN BIOTECH (BEIJING) CO., LTD Cat# DP419 HiScript II Q RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat# R223-01 HiScript II One Step qPT-PCR SYBR Green Kit Vazyme Cat# Q221-00, Lot: 7E750A3 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 DAB Horseradish Peroxidase Color Development Kit Fuzhou Maixin Biotech.
Control:Article Title: Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep anti-SULT1B1 R&D Systems Cat# AF5959; RRID: AB_10571701; Lot:CDWJD12104A Rabbit anti-SULT1B1 Thermo Fisher Scientific Cat# PA5-50796; RRID: AB_2636248; Lot: 30FB4A02 Rabbit anti-H3Y99 Sulfation PTM Biolabs Cat# CTM-211; Lot: ZC199K415P2 Mouse anti-α-Tubulin Abclonal Cat# AC012; RRID: AB_2768341; Lot: 9100012001 Rabbit anti-Histone H3 Abclonal Cat# A2348; RRID: AB_2631273; Lot: 35075 Rabbit anti-PAPSS2 Proteintech Cat# 15978-1-AP; RRID: AB_2159498; Lot: 00041398 Rabbit anti-PAPSS1 Proteintech Cat# 14708-1-AP; RRID: AB_2159487; Lot: 00046631 Rabbit anti-SLC26A1 HUABIO Cat# TP50897; Lot: RB8023 Guinea pig anti-NeuN Oasis biofarm Cat# OB-PGP006-01; RRID: AB_2934234 Guinea pig anti-iba1 Oasis biofarm Cat# OB-PGP049-01; RRID: AB_2934253 Guinea pig anti-GFAP Oasis biofarm Cat# PGP055; RRID: AB_2938933 Guinea pig anti-Olig2 Oasis biofarm Cat# OB-PGP040-02; RRID: AB_2934243 Goat anti-CD31 R&D Systems Cat# AF3628; RRID: AB_2161028 Rabbit anti-ARG1 CST Cat# 93668; RRID: AB_2800207 Rabbit anti-CD86 CST Cat# 19589; RRID: AB_2892094 Goat anti-Guinea pig IgG, 488nm Oasis biofarm Cat# OB-GP488-50; RRID: AB_3717497 Donkey anti-Guinea pig IgG, 594nm Oasis biofarm Cat# D-GP594-50; RRID: AB_3694202 Alexa Fluor®594 donkey anti-rabbit IgG(H + L) Thermo Fisher Cat# A21207; RRID: AB_141637 Alexa Fluor®488 donkey anti-goat IgG(H + L) Thermo Fisher Cat# A11055; RRID: AB_2534102 Rabbit anti-PDK1 Abclonal Cat# A8930; RRID: AB_2863629, Lot: 4000001355 Mouse anti-PFKP Proteintech Cat# 68129-1-Ig; RRID: AB_2923656, Lot: 10029401 Mouse anti-PKM2 Proteintech Cat# 60268-1-Ig; RRID: AB_2881388, Lot: 10024075 Mouse anti-HK2 ProMab Cat# 30088 Bacterial and virus strains Trans1-T1 Phage Resistant Chemically Competent Cell TransGen Biotech Cat# CD501-02 pAAV[shRNA]-CAG>EGFP:T2A:Puro-U6>mPapss1 VectorBuilder Inc. N/A pAAV[shRNA]-EGFP-U6>Scramble_shRNA VectorBuilder Inc. N/A pAAV[Exp]-CAG>mPapss1[NM_011863.3](ns): P2A:EGFP:WPRE VectorBuilder Inc. N/A pAAV[Exp]-CAG>EGFP:WPRE VectorBuilder Inc. N/A Biological samples Human serum samples from patients with ischemic stroke The Affiliated Nanhua Hospital, University of South China N/A Human serum samples from healthy control subjects The Affiliated Nanhua Hospital, University of South China N/A (Continued on next page) e1 Cell Reports Medicine 7, 102684, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMSO VWR Chemicals Cat# 0231-500ML; CAS: 67-68-5, Lot: 22D2056114 Tween 20 BioFroxx Cat# 1247ML500 Fetal bovine serum Pricella Cat# 086–150; Lot: SA240119 DMEM basic (1X) Gibco Cat# SH30285.01; Lot: 6124583 Penicillin-Streptomycin Solution (100X) biosharp Cat# BL505A; Lot: 24208424 0.25% Trypsin-EDTA (1X) Gibco Cat# 25200-072, Lot: 2919428 Phosphatase inhibitor cocktail I MCE Chemexpress Cat# HY-K0021 Phosphatase inhibitor cocktail II MCE Chemexpress Cat# HY-K0022 Protease inhibitor cocktail MCE Chemexpress Cat# HY-K0010 Deacetylase inhibitor cocktail MCE Chemexpress Cat# HY-K0030 Coomassie brilliant blue R-250 BioFroxx Cat# 1912 Trypan Blue Staining Solution (0.4%) Biosharp Cat# BL627A, Lot: 23338403 Methylsulfonylmethane Aladdin CAS: 67-71-0 AgeI-HF BioLabs Cat# R3552L EcoRI-HF BioLabs Cat# R3101S Polyethylenimine Linear (PEI) Yeasen Biotechnology Cat# 40815ES03 2x Phanta Max Master Mix Vazyme Cat# P515-02, Lot: 7E2352K4 Critical commercial assays E.Z.N.A Plasmid Mini Kit I Omega Bio-tek Cat# D6943-02 RNAsimple Total RNA Kit TIANGEN BIOTECH (BEIJING) CO., LTD Cat# DP419 HiScript II Q RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat# R223-01 HiScript II One Step qPT-PCR SYBR Green Kit Vazyme Cat# Q221-00, Lot: 7E750A3 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 DAB Horseradish Peroxidase Color Development Kit Fuzhou Maixin Biotech.
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